The University of Toronto Biosafety Program is designed to protect lab personnel and the public from potential exposure to biological materials used within the University and to prevent unintentional release into the environment. The program includes a number of components, including laboratory containment requirements (physical and operational controls); medical surveillance (including immunization where appropriate); classroom and online training; and compliance with regulatory requirements for importing and shipping biological material [i.e. viruses, bacteria, fungi, prions, toxins, viral vectors, recombinant DNA (rDNA), synthetic DNA (sDNA), microorganisms, parasites, human/animal tissues, cell lines and cell cultures, blood and bodily fluids from humans and animals].
Overall control of the Program is provided by the University’s Institutional Biosafety Committee (IBC), with administrative and technical support provided by the Biosafety Team in EHS.
The Canadian Biosafety Standards and Canadian Biosafety Handbook should be used as a reference for general biosafety and biosecurity practices. Other standards may apply based on the biological materials used and research projects (e.g. CFIA Containment Standards for Facilities Handling Aquatic Animal Pathogens, Containment Standards for Facilities Handling Plant Pests). Other containment standards and guidelines from Environment and Climate Change Canada may be required for genetically modified invertebrates and plants, or invasive species. This Manual is specific to the University of Toronto’s practices and operations.
Emergency Procedures
Reporting of Accidents, Incidents and Occupational Illnesses
Institutional Biosafety & Biosecurity Committee (IBBC)
Responsibilities
Safety Practice and Procedures
- Biosafety Training
- Biological Spill Kit
- Decontamination and Disinfection agents (chemical, irradiation)
- General Biological Spill Procedures
- General Laboratory Safety Practices
- Minimizing Aerosols
- Vacuum Line Set-up
- Using Needles and Syringes
- Principles of Sterilization, Disinfection, and Decontamination
- Pathogen Safety Data Sheets
- Procedure for Cleanup of Laboratory Surfaces Contaminated With Ethidium Bromide (PDF)
- Working with Human Pathogens
- Working with Laboratory Animals
- Working With Doxorubicin-Treated Animals (PDF)
- Working with Semicarbazide Hydrochloride-Treated Animals (PDF)
- Working with Temozolomide-Treated Animals (PDF)
- Working with Chlorpyrifos and Chlorpyrifos-Treated Animals (PDF)
- Working with Tamoxifen and Tamoxifen treated animals (PDF)
Laboratory Waste Management
Laboratory Equipment
Medical Surveillance and Immunoprophylaxis
Importation, Use and Distribution of Biological Materials
Laboratory Decommissioning
Laboratory Standards
- Canadian Biosafety Standards
- Containment Standards for Facilities Handling Plant Pests
- Containment Standards for Facilities Handling Aquatic Animal Pathogens
Additional Resources:
- Public Health Agency of Canada
- Canadian Food Inspection Agency
- Biosafety in Microbiological and Biomedical Laboratories (BMBL)
- World Health Organization (WHO) Laboratory Biosafety Manual
- European Centre for Disease Prevention and Control
- National Institutes of Health (NIH)
- American Biological Safety Association (ABSA)
- Health Protection Agency (UK)
- National Institute of Allergy and Infectious Diseases (NIAID)
- Belgian Biosafety Server
- American Type Culture Collection (ATCC)
- Biosafety Considerations for Research with Lentiviral Vectors (NIH)
- Selected EPA-registered Disinfectants
- CDC – Storage and Shipping of Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae
- IATA Dangerous Goods Documentation
- IATA section 3.6.2 Division 6.2—Infectious Substances
- Transport Canada – Guide to Category A and Category B Assignment SOR/2012-245